Molecular biological analyses are carried out using PCR and real-time PCR methods. PCR, i.e. polymerase chain reaction is a method to detect nucleic acids (in other words, genetic material) of the pathogen. Unlike many traditional diagnostic methods that detect indirect infection signs, the PCR method can identify a unique DNA fragment that is specific only to the pathogen in the test material. PCR diagnostics is especially effective in detecting difficult-to-culture (for example, ureaplasma) and non-culturable (viruses, chlamydia, etc.) microorganisms that are often encountered in latent and chronic infections. The starting material for PCR can be obtained from any tissue or fluids.